For Research Use Only. Available to healthcare professionals and research institutions.
EXORPTION – Extracellular Vesicle (EV) Purification Kit
EXORPTION™ Extracellular Vesicle (EV) Purification Kit
What Makes EXORPTION™ Exceptional?
1. Quick & Simple EV Purification in Under 90 Minutes
EXORPTION™ is an advanced EV purification kit developed by Sanyo Chemical Industries. It enables easy, high-purity extraction of EVs with a high recovery rate—all within just 90 minutes. Utilizing proprietary hydrogel bead spin columns, purification is done with a standard benchtop centrifuge—no need for ultracentrifugation or specialized equipment.
- ~1/20 the purification time of ultracentrifugation
- <1/100 foreign impurities
- ~10x higher recovery yield
2. Superior Recovery & Purity Compared to Ultracentrifugation and Other Kits
EXORPTION™ delivers >10x higher EV recovery than the conventional “gold standard” ultracentrifugation method—perfect for analyzing low-EV biofluids or harvesting large quantities for therapeutic use. Unlike affinity methods, EXORPTION™ avoids residual chemicals and maintains EV membrane integrity, making it highly suited for drug discovery and downstream applications.
Comparison of EV Bioseparation Methods
Several EV purification techniques were evaluated, including newer methods designed to simplify and accelerate the process compared to the traditional ultracentrifugation approach. The table below summarizes each method’s mechanism, processing time, and key performance features.
Method
Mechanism
Processing Time
Performance & Features
Ultracentrifugation
Separates EVs using centrifugal force into distinct fractions (e.g., pellet-down and density gradient centrifugation).
1–3 days
Considered the gold standard in EV research. However, it has low recovery efficiency and purity. Results may vary due to operator skill. No risk of reagent contamination.
Filtration
Adds a polymer reagent to induce EV aggregation and precipitation from biological fluids.
30–120 minutes
Simple and low-labor process. However, EVs tend to adhere to membranes, causing significant loss and making high-purity separation challenging.
Precipitation
Adds a polymer reagent to induce EV aggregation and precipitation from biological fluids.
30–120 minutes
Fast and easy. High yield, but foreign impurities often co-precipitate. Final product may be contaminated with residual reagents.
Affinity
Uses antibody- or ligand-coated carriers to bind EVs selectively, followed by washing and elution.
A few hours
High specificity and purity. However, scalability is limited, and residual reagents may remain depending on the elution method.
HAS (Hydrogel Adsorption Separation)
EVs bind to the surface of hydrogel beads while small contaminants are absorbed into the gel and larger ones remain outside.
70–90 minutes
Offers high purity and recovery in a short time. Simple operation with minimal manual steps. No reagent contamination; maintains EV integrity.
The HAS Method – Hydrogel Adsorption Separation
Developed in collaboration with the University of Tokushima, the HAS method uses specially designed hydrogel beads that:
- Absorb and trap EVs at the surface
- Filter out small molecules into the gel matrix
- Wash away high-molecular-weight contaminants
- Elute intact EVs using mild buffer conditions
This process enables gentle, reagent-free purification while maintaining EV structural and functional integrity.
EV Purification with EXORPTION – Real Sample Results
Tested Samples:
- Human Urine
- Serum
- Plasma
- Cell Culture Supernatant (HEK293)
Analysis Performed:
- Nanoparticle Tracking Analysis (NTA)
- Western Blot / ELISA (CD9, CD63, CD81)
- RNA Quantification & RT-qPCR
- Proteomics
- Single Vesicle Analysis (Flow Cytometry)
Nanoparticle Tracking Analysis (NTA) Summary
Images
Sample
Mean Diameter (nm)
Mode Diameter (nm)
Human Urine
109.8 ± 1.2
73.8 ± 2.1
Human Serum
103.2 ± 1.9
78.5 ± 2.3
Human Plasma
84.4 ± 0.8
70.8 ± 0.8
HEK293 Culture Media
110.5 ± 0.8
85.4 ± 4.0
Key Results:
Robust marker detection across sample types (CD9, CD63, CD81)
Quantification of CD9+/CD63+ EV by ELISA (CD9/CD63)
Western Blot
Consistent miRNA and RNA recovery
Total RNA in EV
RT-PCR
Verified presence of EV proteins (e.g., HLA-A/B, GPC1)
Proteome analysis results
GO Enrichment Analysis Results
Clear distinction of small vs. large EVs in single vesicle analysis
Single vesicle analysis with BD FACSymphony™ A1
EXORPTION - Product Information
Kit Contents:
- Purification Columns ×10
- LureCAP ×10
- Wash Buffer (×10) 1.7mL ×1
- Elution Buffer 1.7mL ×1
- Dilution Container ×1
Storage: Room temperature
Required Equipment:
- Pipettes & low adsorption tips
- 2mL microtubes
- Tabletop centrifuge
- Vortex mixer
- Cooled centrifuge (optional)
Questions Asked About EXORPTION and Other FAQs
EVs are nano-sized particles secreted by cells that carry proteins, lipids, and nucleic acids. Their functions range from cell-to-cell communication to disease signaling and therapeutic effects. Key EV subtypes include exosomes, microvesicles, and apoptotic bodies, collectively referred to as “EVs” by ISEV (International Society for Extracellular Vesicles).
EVs are being investigated for:
- Early disease diagnostics (e.g., cancer, neurodegeneration)
- Cell-free therapeutics (e.g., inflammation reduction, organ regeneration)
EV-based therapies face technical hurdles:
- Standardization of raw materials (cell source, culture media)
- Effective and scalable purification methods
- Safety and regulatory clarity
- Identification and quantification of active ingredients
EXORPTION addresses key separation and purification issues with a novel bioseparation solution.
Urine, plasma, serum, and culture supernatant.
About 70–90 minutes.
Yes, filtration or centrifugation to remove debris is recommended.
Typically 1 mL (or 0.2 mL for plasma/serum).
Yields are approximately 10x greater than ultracentrifugation.
No. The spin columns are for single use only.
Use immediately or store under appropriate conditions; avoid repeated freeze-thaw cycles.
For product inquiries, technical support, or sample requests: